Development of a Forensic Multiplex Amplification STR Kit for 15 Autosomal STR Loci and 10 Y-STR Loci / 法医学杂志

OBJECTIVE@#To establish a multiplex STR genotyping method for autosomal STR and Y-STR loci in forensic biological practice.@*METHODS@#Widely used autosomal STR loci and Y-STR loci were selected. A set of PCR primers was designed, and a 5-dye fluorescent labeled STR multiplex PCR reagent kit was developed.@*RESULTS@#A kit was developed which can simultaneously detect 15 autosomal STR loci, 10 Y-STR loci, and an Amelogenin.@*CONCLUSION@#The 15 autosomal STR plus 10 Y-STR kit in combination with capillary electrophoresis method was used to STR genotyping with accurate and reliable results. The new one-step testing kit can potentially be widely used in forensic cases and DNA databank in the future.

Forensic application of expressmarker 22 STR loci direct PCR amplification kit / 法医学杂志

OBJECTIVE@#To explore the application value of Expressmarker 22 STR loci direct PCR amplification kit.@*METHODS@#One thousand nine hundred and forty-eight samples (including samples spotted on FTA cards, filter papers and case samples) were tested using Expressmarker 22 STR loci direct PCR amplification kit. At the same time, all were tested using Sinofiler kit, Identifiler kit and PowerPlex 16 kit respectively for comparison. The genotypes were compared at the same STR loci among these four kits to test the sensitivity and accuracy of Expressmarker 22 STR loci direct PCR amplification kit.@*RESULTS@#97.79% samples were successfully typed using Expressmarker 22 STR loci direct PCR amplification kit. The genotype profiles of the same samples using Expressmarker 22 STR loci direct PCR amplification kit were consistent with Sinofiler kit, Identifiler kit and PowerPlex 16 kit at the same STR loci.@*CONCLUSION@#Expressmarker 22 STR loci direct PCR amplification kit can provide huge information and accurate results

Rapid DNA identification using 6+1 STR kit and EX-Q20 electrophoresis / 法医学杂志

OBJECTIVE@#To establish a rapid STR genotyping method for individual identification.@*METHODS@#Two hundred blood samples from FTA were collected. Equal amount of blood were collected by puncher and analyzed using two methods (6+1 STR kit in combination with EX-Q20 electrophoresis and Sinofiler kit in combination with POP4 electrophoresis). Consuming time and results of two methods were compared.@*RESULTS@#6+1 STR kit in combination with EX-Q20 electrophoresis method can obtain all genotyping results and be shorter time.@*CONCLUSION@#6+1 STR kit in combination with EX-Q20 electrophoresis method is used to STR genotyping with accurate, reliable results and this new method is potential value in mass personnel investigation and comparison in major criminal cases. It also can raise the work efficiency.

Treatment of 58 cases of vitiligo by electro-plum-blossom needle therapy combined with catgut implantation at acupoints under TDP radiation / 中国针灸

<p><b>OBJECTIVE</b>To search for a method for increasing therapeutic effect on vitiligo.</p><p><b>METHODS</b>One hundred and sixteen cases were randomly divided into a treatment group and a control group, 58 cases in each group. The treatment group were treated with electro-plum-blossom needle therapy plus catgut implantation at local acupoints under TDP radiation, and the control group with external application of sicorten. Their short-term and long-term therapeutic effects were observed.</p><p><b>RESULTS</b>The short-term total effective rate was 98.3% in the treatment group and 74.1% in the control group with a significant difference between the two groups (P < 0.05).</p><p><b>CONCLUSION</b>Electro-plum-blossom needle therapy plus catgut implantation at acupoints has a better therapeutic effect on vitiligo with no adverse effect.</p>